mouse il-4 biotinylated antibody Search Results


biotinylated antibodies against interleukin 1 il 1 α  (R&D Systems)
93
R&D Systems biotinylated antibodies against interleukin 1 il 1 α
Biotinylated Antibodies Against Interleukin 1 Il 1 α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il-4+biotinylated+antibody/Mouse+IL-4+Biotinylated+Antibody/pm36467652-74-10-15
Average 93 stars, based on 1 article reviews
biotinylated antibodies against interleukin 1 il 1 α - by Bioz Stars, 2026-10
93/100 stars
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biotinylated anti mouse il 4 antibody  (R&D Systems)
92
R&D Systems biotinylated anti mouse il 4 antibody
a Protocol outline. Mice were vaccinated <t>with</t> <t>IL-4-K</t> and/or IL-13-K (or PBS or CRM 197 as controls), combined with the adjuvant SWE. At day 39, mice were sensitized and challenged with HDM or PBS, as indicated. b , c Anti-IL-4 ( b ) and anti-IL-13 ( c ) neutralizing capacity in sera collected at the indicated time points. Data show values from individual mice ( n = 12/group) with bars indicating median, from a single experiment representative of three independent experiments. d , e Lung resistance ( d ) and elastance ( e ) in response to inhaled methacholine 24 h after the last HDM challenge. Data represent mean ± SEM from two independent experiments ( n = 8 mice in IL-4-K + IL-13-K/HDM group; n = 9 mice in PBS/PBS and CRM 197 /HDM groups; or n = 10 mice in CRM 197 /PBS, IL-4-K/HDM, and IL-13-K/HDM groups). f , g Numbers of eosinophils in BAL fluid ( f ) and lung tissue ( g ) 24 h after the last HDM challenge. Data show values from individual mice ( n = 7 mice for HDM/CRM 197 group and n = 8 mice for all other groups) with bars indicating mean ± SEM, from a single experiment representative of two independent experiments. h Levels of IL-5 in BAL fluid 24 h after the last challenge. Data show values from individual mice ( n = 8/group pooled from two independent experiments) with bars indicating mean ± SEM. i , j Representative lung sections stained with hematoxylin and eosin (H&E; revealing leukocyte infiltration) ( i ), or periodic acid–Schiff (PAS; revealing mucus-producing goblet cells in dark purple) ( j ). Lower panels in i and j represent magnifications of the dashed areas. Lung sections are representative of each group ( n = 8 mice/group). P values in d and e were calculated using two-way ANOVA followed by a Tukey posttest. d : ### < 0.0001, # = 0.0381, ***: 0.003 for IL-13-K/HDM, 0.0002 for IL-4-K + IL-13-K/HDM, <0.0001 for CRM 197 /PBS and PBS/PBS; e : ### < 0.0001, ** = 0.0019, ***: <0.0001 for all groups. P values in f – h were calculated using two-tailed Mann–Whitney U test ( f – h ) vs. CRM 197 /PBS ( f , g ), CRM 197 /HDM group ( h ), or indicated groups. Source data are provided in the file.
Biotinylated Anti Mouse Il 4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il-4+biotinylated+antibody/Mouse+IL-4+Biotinylated+Antibody/pmc08113315-204-7-14
Average 92 stars, based on 1 article reviews
biotinylated anti mouse il 4 antibody - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier
biotinylated anti-mouse-anti-ifn-gamma or -il-4 detection antibodies  (Becton Dickinson)
90
Becton Dickinson biotinylated anti-mouse-anti-ifn-gamma or -il-4 detection antibodies
a Protocol outline. Mice were vaccinated <t>with</t> <t>IL-4-K</t> and/or IL-13-K (or PBS or CRM 197 as controls), combined with the adjuvant SWE. At day 39, mice were sensitized and challenged with HDM or PBS, as indicated. b , c Anti-IL-4 ( b ) and anti-IL-13 ( c ) neutralizing capacity in sera collected at the indicated time points. Data show values from individual mice ( n = 12/group) with bars indicating median, from a single experiment representative of three independent experiments. d , e Lung resistance ( d ) and elastance ( e ) in response to inhaled methacholine 24 h after the last HDM challenge. Data represent mean ± SEM from two independent experiments ( n = 8 mice in IL-4-K + IL-13-K/HDM group; n = 9 mice in PBS/PBS and CRM 197 /HDM groups; or n = 10 mice in CRM 197 /PBS, IL-4-K/HDM, and IL-13-K/HDM groups). f , g Numbers of eosinophils in BAL fluid ( f ) and lung tissue ( g ) 24 h after the last HDM challenge. Data show values from individual mice ( n = 7 mice for HDM/CRM 197 group and n = 8 mice for all other groups) with bars indicating mean ± SEM, from a single experiment representative of two independent experiments. h Levels of IL-5 in BAL fluid 24 h after the last challenge. Data show values from individual mice ( n = 8/group pooled from two independent experiments) with bars indicating mean ± SEM. i , j Representative lung sections stained with hematoxylin and eosin (H&E; revealing leukocyte infiltration) ( i ), or periodic acid–Schiff (PAS; revealing mucus-producing goblet cells in dark purple) ( j ). Lower panels in i and j represent magnifications of the dashed areas. Lung sections are representative of each group ( n = 8 mice/group). P values in d and e were calculated using two-way ANOVA followed by a Tukey posttest. d : ### < 0.0001, # = 0.0381, ***: 0.003 for IL-13-K/HDM, 0.0002 for IL-4-K + IL-13-K/HDM, <0.0001 for CRM 197 /PBS and PBS/PBS; e : ### < 0.0001, ** = 0.0019, ***: <0.0001 for all groups. P values in f – h were calculated using two-tailed Mann–Whitney U test ( f – h ) vs. CRM 197 /PBS ( f , g ), CRM 197 /HDM group ( h ), or indicated groups. Source data are provided in the file.
Biotinylated Anti Mouse Anti Ifn Gamma Or Il 4 Detection Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il-4+biotinylated+antibody/biotinylated+anti+mouse+anti+ifn+gamma+or++il+4+detection+antibodies/us09463228-318-70-76
Average 90 stars, based on 1 article reviews
biotinylated anti-mouse-anti-ifn-gamma or -il-4 detection antibodies - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


a Protocol outline. Mice were vaccinated with IL-4-K and/or IL-13-K (or PBS or CRM 197 as controls), combined with the adjuvant SWE. At day 39, mice were sensitized and challenged with HDM or PBS, as indicated. b , c Anti-IL-4 ( b ) and anti-IL-13 ( c ) neutralizing capacity in sera collected at the indicated time points. Data show values from individual mice ( n = 12/group) with bars indicating median, from a single experiment representative of three independent experiments. d , e Lung resistance ( d ) and elastance ( e ) in response to inhaled methacholine 24 h after the last HDM challenge. Data represent mean ± SEM from two independent experiments ( n = 8 mice in IL-4-K + IL-13-K/HDM group; n = 9 mice in PBS/PBS and CRM 197 /HDM groups; or n = 10 mice in CRM 197 /PBS, IL-4-K/HDM, and IL-13-K/HDM groups). f , g Numbers of eosinophils in BAL fluid ( f ) and lung tissue ( g ) 24 h after the last HDM challenge. Data show values from individual mice ( n = 7 mice for HDM/CRM 197 group and n = 8 mice for all other groups) with bars indicating mean ± SEM, from a single experiment representative of two independent experiments. h Levels of IL-5 in BAL fluid 24 h after the last challenge. Data show values from individual mice ( n = 8/group pooled from two independent experiments) with bars indicating mean ± SEM. i , j Representative lung sections stained with hematoxylin and eosin (H&E; revealing leukocyte infiltration) ( i ), or periodic acid–Schiff (PAS; revealing mucus-producing goblet cells in dark purple) ( j ). Lower panels in i and j represent magnifications of the dashed areas. Lung sections are representative of each group ( n = 8 mice/group). P values in d and e were calculated using two-way ANOVA followed by a Tukey posttest. d : ### < 0.0001, # = 0.0381, ***: 0.003 for IL-13-K/HDM, 0.0002 for IL-4-K + IL-13-K/HDM, <0.0001 for CRM 197 /PBS and PBS/PBS; e : ### < 0.0001, ** = 0.0019, ***: <0.0001 for all groups. P values in f – h were calculated using two-tailed Mann–Whitney U test ( f – h ) vs. CRM 197 /PBS ( f , g ), CRM 197 /HDM group ( h ), or indicated groups. Source data are provided in the file.

Journal: Nature Communications

Article Title: Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice

doi: 10.1038/s41467-021-22834-5

Figure Lengend Snippet: a Protocol outline. Mice were vaccinated with IL-4-K and/or IL-13-K (or PBS or CRM 197 as controls), combined with the adjuvant SWE. At day 39, mice were sensitized and challenged with HDM or PBS, as indicated. b , c Anti-IL-4 ( b ) and anti-IL-13 ( c ) neutralizing capacity in sera collected at the indicated time points. Data show values from individual mice ( n = 12/group) with bars indicating median, from a single experiment representative of three independent experiments. d , e Lung resistance ( d ) and elastance ( e ) in response to inhaled methacholine 24 h after the last HDM challenge. Data represent mean ± SEM from two independent experiments ( n = 8 mice in IL-4-K + IL-13-K/HDM group; n = 9 mice in PBS/PBS and CRM 197 /HDM groups; or n = 10 mice in CRM 197 /PBS, IL-4-K/HDM, and IL-13-K/HDM groups). f , g Numbers of eosinophils in BAL fluid ( f ) and lung tissue ( g ) 24 h after the last HDM challenge. Data show values from individual mice ( n = 7 mice for HDM/CRM 197 group and n = 8 mice for all other groups) with bars indicating mean ± SEM, from a single experiment representative of two independent experiments. h Levels of IL-5 in BAL fluid 24 h after the last challenge. Data show values from individual mice ( n = 8/group pooled from two independent experiments) with bars indicating mean ± SEM. i , j Representative lung sections stained with hematoxylin and eosin (H&E; revealing leukocyte infiltration) ( i ), or periodic acid–Schiff (PAS; revealing mucus-producing goblet cells in dark purple) ( j ). Lower panels in i and j represent magnifications of the dashed areas. Lung sections are representative of each group ( n = 8 mice/group). P values in d and e were calculated using two-way ANOVA followed by a Tukey posttest. d : ### < 0.0001, # = 0.0381, ***: 0.003 for IL-13-K/HDM, 0.0002 for IL-4-K + IL-13-K/HDM, <0.0001 for CRM 197 /PBS and PBS/PBS; e : ### < 0.0001, ** = 0.0019, ***: <0.0001 for all groups. P values in f – h were calculated using two-tailed Mann–Whitney U test ( f – h ) vs. CRM 197 /PBS ( f , g ), CRM 197 /HDM group ( h ), or indicated groups. Source data are provided in the file.

Article Snippet: After incubation, bound kinoids were detected using biotinylated anti-mouse IL-4 antibody (polyclonal goat IgG, R&D systems, BAF-404), biotinylated anti-human IL-4 antibody (polyclonal goat IgG, R&D systems, BAF204), biotinylated anti-mouse IL-13 antibody (polyclonal goat IgG, R&D systems, BAF-413), or biotinylated anti-human IL-13 antibody (polyclonal goat IgG, R&D systems, BAF213) at 250 ng/ml, and then revealed with streptavidin-HRP and an OPD substrate.

Techniques: Adjuvant, Staining, Two Tailed Test, MANN-WHITNEY

a – c Levels of total IgE ( a ), HDM-specific IgE ( b ), and HDM-specific IgG ( c ) 24 h after the last HDM challenge. Results show values from individual mice with bars indicating mean ± SEM from n = 12 mice (PBS/PBS group), n = 16 mice (CRM 197 /PBS group), n = 19 mice (IL-13-K/HDM group), or n = 20 mice (IL-4-K and IL-4-K + IL-13-K groups) pooled from two independent experiments. d Representative lung sections stained with toluidine blue, demonstrating mast cells (arrows) 24 h after the last HDM challenge. Insert represent magnifications of the dashed areas. e Quantification of toluidine blue-positive lung mast cells. Results show values from individual mice with bars indicating mean ± SEM from n = 8 mice. f Representative lung sections stained with avidin (which stains mast cells, red), anti-IgE (green), and DAPI (blue) 24 h after the last HDM challenge. g Quantification of IgE levels in avidin-positive lung mast cells. Results show values from individual avidin-positive mast cells analyzed in ear skin sections from n = 4 mice/group with bars indicating means ± SEMs. P values were calculated using two-tailed Mann–Whitney U test vs. PBS/PBS group (in a ), CRM 197 /PBS group (in b , e , and g ) or vs. indicated groups. Source data are provided in the file.

Journal: Nature Communications

Article Title: Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice

doi: 10.1038/s41467-021-22834-5

Figure Lengend Snippet: a – c Levels of total IgE ( a ), HDM-specific IgE ( b ), and HDM-specific IgG ( c ) 24 h after the last HDM challenge. Results show values from individual mice with bars indicating mean ± SEM from n = 12 mice (PBS/PBS group), n = 16 mice (CRM 197 /PBS group), n = 19 mice (IL-13-K/HDM group), or n = 20 mice (IL-4-K and IL-4-K + IL-13-K groups) pooled from two independent experiments. d Representative lung sections stained with toluidine blue, demonstrating mast cells (arrows) 24 h after the last HDM challenge. Insert represent magnifications of the dashed areas. e Quantification of toluidine blue-positive lung mast cells. Results show values from individual mice with bars indicating mean ± SEM from n = 8 mice. f Representative lung sections stained with avidin (which stains mast cells, red), anti-IgE (green), and DAPI (blue) 24 h after the last HDM challenge. g Quantification of IgE levels in avidin-positive lung mast cells. Results show values from individual avidin-positive mast cells analyzed in ear skin sections from n = 4 mice/group with bars indicating means ± SEMs. P values were calculated using two-tailed Mann–Whitney U test vs. PBS/PBS group (in a ), CRM 197 /PBS group (in b , e , and g ) or vs. indicated groups. Source data are provided in the file.

Article Snippet: After incubation, bound kinoids were detected using biotinylated anti-mouse IL-4 antibody (polyclonal goat IgG, R&D systems, BAF-404), biotinylated anti-human IL-4 antibody (polyclonal goat IgG, R&D systems, BAF204), biotinylated anti-mouse IL-13 antibody (polyclonal goat IgG, R&D systems, BAF-413), or biotinylated anti-human IL-13 antibody (polyclonal goat IgG, R&D systems, BAF213) at 250 ng/ml, and then revealed with streptavidin-HRP and an OPD substrate.

Techniques: Staining, Avidin-Biotin Assay, Two Tailed Test, MANN-WHITNEY

a Protocol outline. Mice were sensitized and challenged with HDM extract (or PBS as a control), as indicated. After the third challenge, mice were vaccinated with IL-4-K and IL-13-K (or CRM 197 as control), combined with the adjuvant SWE. b , c Anti-IL-4 ( b ) and anti-IL-13 ( c ) neutralizing capacity in sera collected at the indicated time points. d , e . Levels of total IgE ( d ) and HDM-specific IgE ( e ) 24 h after the last HDM challenge. f , g Lung resistance ( f ) and elastance ( g ) in response to inhaled methacholine 24 h after the last HDM challenge. h Eosinophil numbers in BAL fluid 24 h after the last HDM challenge. i Representative periodic acid–Schiff (PAS) staining of lung sections, demonstrating mucus-producing goblet cells (dark purple). j Quantification of mucus-producing goblet cells. Data show median ( b , c ) or mean ± SEM ( d – h , j ) from n = 4 ( j ), 7 ( f , g ), or 12 ( b – e ) mice in the PBS groups, and n = 8 ( j ) or 14 ( b – e ) mice in the HDM groups pooled from two independent experiments. Each symbol represents individual mice ( b – e , h , j ). P values were calculated using two-tailed Mann–Whitney U test in b – e , h , and j , or two-way ANOVA followed by a Tukey posttest in f and g . Source data are provided in the file.

Journal: Nature Communications

Article Title: Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice

doi: 10.1038/s41467-021-22834-5

Figure Lengend Snippet: a Protocol outline. Mice were sensitized and challenged with HDM extract (or PBS as a control), as indicated. After the third challenge, mice were vaccinated with IL-4-K and IL-13-K (or CRM 197 as control), combined with the adjuvant SWE. b , c Anti-IL-4 ( b ) and anti-IL-13 ( c ) neutralizing capacity in sera collected at the indicated time points. d , e . Levels of total IgE ( d ) and HDM-specific IgE ( e ) 24 h after the last HDM challenge. f , g Lung resistance ( f ) and elastance ( g ) in response to inhaled methacholine 24 h after the last HDM challenge. h Eosinophil numbers in BAL fluid 24 h after the last HDM challenge. i Representative periodic acid–Schiff (PAS) staining of lung sections, demonstrating mucus-producing goblet cells (dark purple). j Quantification of mucus-producing goblet cells. Data show median ( b , c ) or mean ± SEM ( d – h , j ) from n = 4 ( j ), 7 ( f , g ), or 12 ( b – e ) mice in the PBS groups, and n = 8 ( j ) or 14 ( b – e ) mice in the HDM groups pooled from two independent experiments. Each symbol represents individual mice ( b – e , h , j ). P values were calculated using two-tailed Mann–Whitney U test in b – e , h , and j , or two-way ANOVA followed by a Tukey posttest in f and g . Source data are provided in the file.

Article Snippet: After incubation, bound kinoids were detected using biotinylated anti-mouse IL-4 antibody (polyclonal goat IgG, R&D systems, BAF-404), biotinylated anti-human IL-4 antibody (polyclonal goat IgG, R&D systems, BAF204), biotinylated anti-mouse IL-13 antibody (polyclonal goat IgG, R&D systems, BAF-413), or biotinylated anti-human IL-13 antibody (polyclonal goat IgG, R&D systems, BAF213) at 250 ng/ml, and then revealed with streptavidin-HRP and an OPD substrate.

Techniques: Adjuvant, Staining, Two Tailed Test, MANN-WHITNEY

a Vaccination protocol outline. hIL-4/hIL-13 KI ; hIL-4Rα KI mice were vaccinated with hIL-4-K and hIL-13-K in combination (or CRM 197 as control), combined with the adjuvant SWE. b , c Anti-human IL-4 ( b ) and anti-human IL-13 ( c ) neutralizing capacity in sera collected at the indicated time point. Results show values from individual mice ( n = 7/group) with bars indicating medians. d , e Levels of total IgE ( d ) in sera or on the surface of blood basophils ( e ) at the indicated time points. Results show values from individual mice ( n = 7/group) with bars indicating means ± SEMs. *, **, or ***: P < 0.05, 0.01 or 0.001 (two-tailed Mann–Whitney U test). f Representative ear skin sections stained with avidin (which stains mast cells, red), anti-IgE (green), and DAPI (blue) 24 h after the last HDM challenge. g Quantification of IgE levels in avidin-positive mast cells. Results show values from individual avidin-positive mast cells analyzed in ear skin sections from n = 7 mice injected with CRM 197 and n = 5 mice injected with hIL-4-K and hIL-13-K, with bars indicating means ± SEMs. P values were calculated using two-tailed Mann–Whitney U test. Source data are provided in the file.

Journal: Nature Communications

Article Title: Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice

doi: 10.1038/s41467-021-22834-5

Figure Lengend Snippet: a Vaccination protocol outline. hIL-4/hIL-13 KI ; hIL-4Rα KI mice were vaccinated with hIL-4-K and hIL-13-K in combination (or CRM 197 as control), combined with the adjuvant SWE. b , c Anti-human IL-4 ( b ) and anti-human IL-13 ( c ) neutralizing capacity in sera collected at the indicated time point. Results show values from individual mice ( n = 7/group) with bars indicating medians. d , e Levels of total IgE ( d ) in sera or on the surface of blood basophils ( e ) at the indicated time points. Results show values from individual mice ( n = 7/group) with bars indicating means ± SEMs. *, **, or ***: P < 0.05, 0.01 or 0.001 (two-tailed Mann–Whitney U test). f Representative ear skin sections stained with avidin (which stains mast cells, red), anti-IgE (green), and DAPI (blue) 24 h after the last HDM challenge. g Quantification of IgE levels in avidin-positive mast cells. Results show values from individual avidin-positive mast cells analyzed in ear skin sections from n = 7 mice injected with CRM 197 and n = 5 mice injected with hIL-4-K and hIL-13-K, with bars indicating means ± SEMs. P values were calculated using two-tailed Mann–Whitney U test. Source data are provided in the file.

Article Snippet: After incubation, bound kinoids were detected using biotinylated anti-mouse IL-4 antibody (polyclonal goat IgG, R&D systems, BAF-404), biotinylated anti-human IL-4 antibody (polyclonal goat IgG, R&D systems, BAF204), biotinylated anti-mouse IL-13 antibody (polyclonal goat IgG, R&D systems, BAF-413), or biotinylated anti-human IL-13 antibody (polyclonal goat IgG, R&D systems, BAF213) at 250 ng/ml, and then revealed with streptavidin-HRP and an OPD substrate.

Techniques: Adjuvant, Two Tailed Test, MANN-WHITNEY, Staining, Avidin-Biotin Assay, Injection